periodic acid schiff pas reagents  (Servicebio Inc)

Journal: The World Allergy Organization Journal

Article Title: Protective effect of micheliolide against inflammation and oxidative stress in asthma through the MAPK/NF-κB signalling pathway ☆

doi: 10.1016/j.waojou.2025.101091

Figure Lengend Snippet: Effect of MCL on airway hyperresponsiveness, airway inflammation, and airway mucus hypersecretion in asthmatic mice. (A) Penh values (an indicator of airway responsiveness) of mice after stimulation with different doses of methacholine across different groups. (B) Counts and classification of inflammatory cells across different groups. (C) Percentage of eosinophils among total inflammatory cells in bronchoalveolar lavage fluid (BALF) across different groups. (D) HE staining of lung tissues across different groups (200 × magnification). (E) Peri-bronchial inflammation scores based on HE staining across different groups. (F) PAS staining of lung tissues in each group (200 × magnification). (G) PAS-positive goblet cell score across different groups based on PAS staining. The Control group received normal saline and DMSO. The OVA group was the asthma model group. The OVA + MCL12.5, OVA + MCL25, and OVA + MCL50 groups were treated with 12.5 mg/kg, 25 mg/kg, and 50 mg/kg of MCL in OVA-induced asthmatic mice, respectively. Data are presented as mean ± standard deviation from 6 independent experiments. Data in A and B were analyzed using two-way ANOVA; data in C, E and G are analyzed using one-way ANOVA. Compared with the Control group: ## p < 0.01; Compared with the OVA group: ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: The lung tissue sections were subjected to staining with a PAS reagent kit (Beyotime, Shanghai, China).

Techniques: Staining, Control, Saline, Standard Deviation